Morphology Characterization of Ten Lytic Bacteriophages from Different Family Targeting Klebsiella pneumoniae

Abeer Amen Baqer, Yip Chee Wai, Siti Noor Adnalizawati Adnan, Norefrina Shafinaz Md Nor

  • Norefrina Shafinaz Md Nor

Abstract

Phage therapy is the therapeutic use of bacteriophages to treat highly drug resistant bacterial infections. Klebsiella pneumonia (KP) has emerged as an increasingly important cause of community-acquired nosocomial infections and many of these strains are highly virulent with multidrug resistance (MDR) ability and exhibit a strong propensity to spread making infection cause by it difficult to treat. Treatment utilizing bacteriophage specific to KP (KP phage) can be used as an alternative counter measure. Characterization of bacteriophage is utmost important in assisting the application of bacteriophage in phage therapy. In the present study, samples from sewage water and cockles were screened for KP phage.  Enrichment detection methods followed by isolation of phage by double agar layers method was done using 9 strains of MDR-KP as host system. KP phages were characterized on the basis of plaque morphology, host range and transmission electron microscope (TEM).  A total of 58 lytic KP phages were isolated from sewage (42) and cockle (16).  Investigation done on host range showed that 52 were KP phage and 6 have broad host range. Further identification by transmission electron microscopy (TEM) was done to 10 selected phages that showed potent lytic activity against MDR-KP. Image from TEM show selected phages were from subfamily caudovirales (7) with icosahedral head and pentagonal structure with short non-contractile tail suggesting it belongs to the family of Podoviridae. Two phages belonged to the family Myoviridae with isometric head and contractile tail and one from the family Siphoviridae with icosahedral and a non-contractile tail. Therefore, this study has partially characterized and revealed 10 potential lytic bacteriophages as therapeutic agent against MDR-KP which mostly resembles Podoviridae family

Published
2021-04-01
Section
Original Research Articles