SUBCHRONIC TOXICITY ANALYSIS OF MIXTURES OF Christia vespertilionis (L.f.) Bakh. f. LEAF AND Morinda citrifolia L. FRUIT ETHANOLIC EXTRACTS IN MALE SPRAGUE DAWLEY RATS
Christia vespertilionis(L.f.) Bakh. f. (CV) leaves and Morinda citrifoa L.(MC) fruits have acquired much attention among Malaysians and are commercially available.Practitioners are concerned about the effectiveness and safety of the products. Thus, the study aims to evaluate the toxicity effects of mixtures of CV and MC in male Sprague Dawley rats in a 90-day subchronic oral toxicity study. A total of 36 rats were divided equally into six groups; control, 5% DMSO (vehicle), mixture of low dose CV and MC (75 mg/kg), mixture of low dose CV (75 mg/kg) and medium-dose MC (125 mg/kg), mixture of medium-dose CV (125 mg/kg) and low dose MC (75 mg/kg) and mixture of medium CV and MC (125 mg/kg). The extracts were orally gavaged daily for 90 days. On day 91, the rats were sacrificed, and the blood profiles and organs changes were evaluated. There was no mortality observed in groups of rats that received mixtures of CV and MC. Weekly body weights, haematology and serum biochemistry also showed no significant (p>0.05) differences compared to control. However, significant (p<0.05) differences were observed for hepatic necrosis and number of activated Kupffer cells in all herbal treated groups compared to control (from very mild to moderate scores). Kidneys showed a significant (p<0.05) score for the granular cast (very mild). In conclusion, mixtures of CV and MC induced hepatotoxicity and renal toxicity, and the no-observed-adverse-effect levels (NOAEL) of CV and MC mixture is lower than 150 mg/kg.