• Ching Theng Koh Faculty of Mechanical and Manufacturing Engineering, Universiti Tun Hussein Onn Malaysia, 86400 Parit Raja, Batu Pahat, Johor, Malaysia
Keywords: Siam weed, Electrospinning, Gelatin, Nanofiber, Tissue Engineering


Siam weed is a traditional herb used for the soothing of burn and scar, and potentially improve healing ability for dressing and tissue-engineered construct. The use of Siam weed is mainly in the form of extract. The form of extract limits the use of Siam weed as compared to the form of gels and membrane. However, the development of the hybrid of Siam weed in gel and membrane form is lacking.  In this study, Siam weed was harvested and make into aqua extracts. The biocompatibility of the aqua extract with various concentration was tested using Cell Proliferation Assay. The aqua extract was then incorporated in the gelatin solution and spun into nanofibers using an electrospinning technique. Cell proliferation study shows maximum cell proliferation at the concentration of 25 μg/ml. The Scanning Electron Microscope images of the electrospun scaffolds shows the microstructures of fibrous networks without beads. The concentration of the hybrid solution was found to affect the morphology of the nanofibers by having diameters in the range of 160 ± 90 to 250 ± 150 nm. The Siam-weed based gelatin electrospun scaffolds provide native-like microenvironment and potentially improve wound healing ability for biomedical application.

Original Research Articles