ADAPTATION, REPLICATION AND ATTENUATION OF VERY VIRULENT INFECTIOUS BURSAL DISEASE VIRUS OF MALAYSIAN ISOLATE IN VERO CELLS:

M Hair-Bejo

M Hair-Bejo

Abstract

ADAPTATION, REPLICATION AND ATTENUATION OF VERY VIRULENT INFECTIOUS BURSAL DISEASE VIRUS OF MALAYSIAN ISOLATE IN VERO CELLS

S.F. Khor, M. Hair-Bejo*, A. R. Omar & I. Aini

Faculty of Veterinary Medicine, Universiti Putra Malaysia

43400 UPM, Serdang, Selangor, Malaysia.

The mammalian continuous cell lines, Vero cells were used for adaptation, replication and attenuation of local isolates of infectious bursal disease virus (IBDV) namely UPM0081. The UPMO081 is a very virulent strain of serotype 1 IBDV. The virus was isolated and propagated in specific pathogen free (SPF) chicken embryonated eggs via chorio-allantoic membrane (CAM) route before adapted in tissue cultures. The cytopathic effect (CPE) formed agents in Vero cells were confirmed as IBDV through indirect immunoperoxidase staining (IIPS). The hyper-variable region of passage 10 (P10) UPM0081 in Vero cells were amplified by reverse transcription polymerase chain reaction (RT-PCR). The VP2 gene of expected size (1.35kb) was successfully isolated and sequenced. The exchange of residue 284 (A to T) was observed.

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E-mail. mdhair@vet.upm.edu.my (M. Hair-Bejo)