DENTAL PLAQUE: CONFOCAL AND SCANNING ELECTRON MICROSCOPY STUDY

K. A. Al-Salihi,* & N. Azura

  • KA Al-Salihi

Abstract

DENTAL PLAQUE: CONFOCAL AND SCANNING ELECTRON MICROSCOPY STUDY

  1. A. Al-Salihi,* & N. Azura

School of Dental Science, Universiti Putra Malaysia. HUSM, PPSG, 16150 Kubang Kerian kelantan Malaysia

The aim of this study was to assess the thickness and content of in vivo accumulated dental plaque on acroflat lower arch splint using confocal laser scanning microscopy (CLSM) combined with vital fluorescence technique and scanning electron microscopy. Accumulated dental plaque revealed varied plaque microflora vitality and thickness according to volunteer's oral hygiene. The thickness of plaque smears ranged from 40.32-140.72um and 65-128.88um for accumulated live (vital) and dead microorganisms respectively, while the thickness of plaque on the appliance ranged from 101um to 653um. Confocal microscopy revealed both dead and vital bacteria on the surface of the dental plaque. Scanning electron microscopy revealed accumulation of different shapes of bacteria in all dental plaque. This study offers a potent non- invasive tool to evaluate and assesse the dental plaque biofilms, which is the effective factor in the development of tooth caries.

Corresponding authour: elsalihi@ yahoo.com elsalihi@kb.usm.my

Published
2015-12-01
Section
Original Research Articles