A SEM INVESTIGATION OF CRUDE AND PEPSIN-SOLUBILIZED COLLAGEN OF SEA CUCUMBER (Bohadschia bivittata)
Y.D. Siddiqui, E.M. Arief*, A. Yusoff, S.S.A. Hamid, N.F. Nik Ali, M.Y.S. Abdullah
AbstractA SEM INVESTIGATION OF CRUDE AND PEPSIN-SOLUBILIZED COLLAGEN
OF SEA CUCUMBER (Bohadschia bivittata)
Y.D. Siddiqui1, E.M. Arief1*, A. Yusoff1, S.S.A. Hamid2, N.F. Nik Ali3, M.Y.S. Abdullah1
1School of Dental Sciences, Health Campus, University Sain Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia.
2School of Medical Sciences, Health Campus, University Sain Malaysia, 16150, Kubang Kerian, Kelantan, Malaysia
3School of Health Sciences, Universiti Sains Malaysia, 16150 Kubang Kerian, Kelantan, Malaysia
Crude and Pepsin-Solubilized Collagen (PSC) has been extracted and isolated from the body wall of Bohadschia bivittata. Sea cucumber body wall contains highly insoluble collagen protein, which becomes soluble after limited digestion with pepsin. A characteristic feature of the collagen fibrils is their banded structure. Collagen types I, II, III, V and XI are types of collagen, self-assemble into D-periodic cross-striated fibrils. Aim of this study was to investigate the morphological feature (banding pattern) of Crude and PSC collagen from body wall of Bohadschia bivittata. The ultrastructures were analyzed by using Quanta™ Scanning Electron Microscope, Netherlands, under 130 Pa pressure, at a working distance of 6-10 mm at 4.0 to 5.0 kV from magnification 5,000X to 100,000X. SEM analysis of Crude collagen from Bohadschia bivittata, showed banded structures on collagen fibrils, whereas PSC collagen fibril was not banded. In conclusion, SEM analysis of crude collagen displayed a regularly repeated striated pattern, whereas, PSC displayed non-banded sheet like fibers with twisted configuration. The banded and not banded features in this species have not been described earlier.
Keywords: Sea cucumber, Bohadschia bivitatta, Pepsin solubilized collagen (PSC), Scanning electron microscope.