HUMAN ADIPOSE TISSUE DERIVED STEM CELLS AS A SOURCE OF SMOOTH MUSCLE CELLS IN THE REGENERATION OF MUSCULAR LAYER OF URINARY BLADDER WALL

Salah Abood Salem , Angela Ng Min Hwei , Christopher C.K Ho , Ismail Sagap , Ruszymah Bt.Hj Idrus , Zulkifili MD Zainuddin*

  • Zulkifili MD Zainuddin

Abstract

HUMAN ADIPOSE TISSUE DERIVED STEM CELLS AS A SOURCE OF SMOOTH MUSCLE CELLS IN THE REGENERATION OF MUSCULAR LAYER OF URINARY BLADDER WALL

 

Salah Abood Salem1,2 , Angela Ng Min Hwei2 , Christopher C.K Ho1,2 , Ismail Sagap1 , Ruszymah Bt.Hj Idrus2,3 , Zulkifili MD Zainuddin1,2

1Department of Surgery, Universiti Kebangsaan Malaysia Medical Centre, Kuala lumpur, Malaysia

2Tissue Engineering Centre, Universiti Kebangsaan Malaysia Medical Centre, Kuala Lumpur, Malaysia

3Department of Physiology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia

 

ABSTRACT

Adipose tissue provides an abundant source of multipotent cells for cell-based regeneration strategies for urinary bladder smooth muscle repair. Our objective is to confirm that adipose derived stem cells (HADSCs) can be differentiated to smooth muscle cells. In this study adipose tissue sample was digested with 0.075% collagenase, and the resulting HADSCs cultured and expanded in vitro. HADSCs at passage 2 was differentiated by incubation in smooth muscle inductive media (SMIM) consisting of MCDB 131 medium, 1% FBS and 100 U/ml heparin. ADSCs in non-inductive media were used as control. Characterization was performed by cell morphology, gene and protein expression. Differentiated cells became elongated and spindle shaped, and towards the end of 6 weeks, sporadic cell aggregation appeared which is typical for smooth muscle cells culture. For gene expression study smooth muscle markers, Alfa smooth muscle actin (ASMA), calponin, and Myocin heavy chain (MHC) were expressed after 3weeks induction. At the protein expression level ASMA, MHC, Smoothelin were expressed after 6 weeks induction. As a conclusion adipose tissue provides a possible source of smooth muscle precursor cells which possess the potential capability for smooth muscle differentiation. This represents a promising alternative for urinary bladder smooth muscle repair.

 

 

Keywords: Adipose Stem cells, Muscle cells, Tissue Engineering, Bladder wall

Published
2013-12-31
Section
Original Research Articles