BACTERIAL MEMBRANE DISRUPTION IN BACILLUS SUBTILIS, STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI BY CYNODON DACTYLON EXTRACT
Syahriel A, Januarius G and Chong K.P*
BACTERIAL MEMBRANE DISRUPTION IN BACILLUS SUBTILIS, STAPHYLOCOCCUS AUREUS AND ESCHERICHIA COLI BY CYNODON DACTYLON EXTRACTSyahriel A1, Januarius G2 and Chong K.P1*
1Sustainable Palm Oil Research Unit (SPOR), Faculty of Science and Natural Resources, Universiti Malaysia Sabah, Jalan UMS, 88400, Kota Kinabalu, Malaysia
2Faculty of Sustainable Agriculture, Universiti Malaysia Sabah, Sandakan Campus, Mile 10, Sg. Batang, 90000, Sandakan, Malaysia
Cynodon dactylon is a type of perennial grass that possesses great medicinal values. It has been reported to possess great antibacterial activity against many Gram positive and negative bacteria. It is believed that most antibacterial compounds show great antibacterial mechanism through membrane toxicity effect. Electron microscope (EM) is a powerful tool in observatory analysis which offers capability to observe extremely small sample or structure up to micron size. In present study the effect of C. dactylon extract on Bacillus subtilis, Staphylococcus aureus and Escherichia coli cellular membrane was investigated using Scanning Electron Microscope (SEM). SEM observation revealed the disruption of bacterial membrane and caused complete lyse in B. subtilis and E. coli and S. aureus after treated with C. dactylon SPE extract which led to bacterial cell death.
Keywords: Cynodon dactylon, membrane disintegration, antibacterial, SPE