PSEUDOMONAS FLUORESCENS BIOFILM DEVELOPMENT AND ITS MICROSCALE INTERACTIONS

Wan Rosmiza Zana WD, Keeffe JO, Robbins PT , Zhibing Z

  • Wan Rosmiza Zana WD

Abstract

PSEUDOMONAS FLUORESCENS BIOFILM DEVELOPMENT AND ITS MICROSCALE INTERACTIONS

Wan Rosmiza Zana WD1,2*, Keeffe JO3, Robbins PT2 , Zhibing Z2

1Faculty of Bioscience and Medical Engineering, UniversitiTeknologi Malaysia, Skudai, Johor.

2School of Chemical Engineering, University of Birmingham, Birmingham, UK

3Unilever R&D, Port Sunlight, Bebington, Wirral, UK.

 

The objective of this work was to investigate the biofilm adhesive strengths on stainless steel substrate using a micromanipulation technique. Initially, the biofilm development was characterized using confocal laser scanning microscopy (CLSM) and the images were quantified using daime software. The biofilm was allowed to grow statically on stainless steel substrate at a temperature of 25°C over a period of 2 to 10 days. The effects of the initial pH (pH 5, pH 7 and pH 9) and initial glucose concentration (0.25, 0.5, 0.75 and 1.0 % (w/v)) on the adhesive strength of biofilm were investigated over the period using a micromanipulation technique. The results revealed that at pH 7 and at a low glucose concentration (0.25 % (w/v)) the adhesion of the biofilm was greatest, particularly at day 6 to day 8.

 

Keywords: biofilm, CLSM, micromanipulation, adhesion

Published
2014-12-31
Section
Original Research Articles