MORPHOLOGICAL CHANGES OF CYTOSKELETAL PROTEINS IN MONOLAYER CELLS OF TISSUE ENGINEERED SKIN

M. N. Monzaii, A.L. Maziyzaml, F. Sharida, R. Asmah, B.S. Aminuddin, B.H.I. Ruszymah, 0. Fauziah*

  • Fauziah Othman

Abstract

MORPHOLOGICAL CHANGES OF CYTOSKELETAL PROTEINS IN MONOLAYER CELLS OF TISSUE ENGINEERED SKIN

  1. N. Monzai1, A.L. Maziyzam2 , F. Sharida1,3, R. Asmah1,3, B.S. Aminuddin2,4 , B.H.I. Ruszymah2,5,0. Fauziah1,3*

'Institute of Bioscience, 2 Tissue Engineering and Human Genetic Lab, 3 Faculty Medicine and Health Sciences, Universiti Putra Malaysia, 4 Hospital Ampang Puteri, 5 Physiology Department, Universiti Kebangsaan Malaysia

Tissue engineering is a field of research which aims at regenerating tissues and organs. During this phenomenon, tissue remodeling or tissue regenerating depends on continuous collagen synthesis by fibroblast cell. In the presence of fibroblast, keratinocyte proliferation is stimulated and epidermal layer well formed. Vimentin and tubulin, the two important proteins in tissue remodeling, play vital role in cell adhesion and spreading. The objective of this study was to observe temporal changes in cell morphology of vimentin and d-tubulin. Morphological changes were studied by using the confocal laser scanning microscopy (CLSM) and observation was done at 1 and 3 weeks post-culture from passage 1. CLSM observation revealed that the d-tubulin and vimentin offibroblast stretched out and high in density with time. Cell has stretched fibers. Simultaneously, the number, size, density and length of cell were increased.

KEYWORDS: Cytoskeleton, cell spreading, a-tubulin and vimentin cytoskeleton

*Corresponding authour:Tel : +6(03)89468439 ; Fax +6(03)86567948 E-mail : fauziah@putra.upm.edu.my

Published
2015-12-01
Section
Original Research Articles