Cytotoxicity and Cell Adherence Evaluation of 3D Orbital Polyamide Composite Customised Implant
Su Bing Sheng, Abdul Manaf Abdullah, Marzuki Omar, Dasmawati Mohamad
Polymer composites based orbital floor implant namely 3D orbital polyamide customised composite (3D OPACC) has been successfully developed locally. The objective of this study was to evaluate the cytotoxicity and osteoblast cell adherence on the developed composite. The cytotoxicity of the 3D OPACC was evaluated by incubating the osteoblast cells in five different concentrations (100, 50, 25, 12.5, 6.25 mg/ml) of the composite extract for 72 hours. The cell viability was determined by MTS assay at a wavelength of 492 nm via a microplate reader. The cell adherence was evaluated by seeding the osteoblast cells on the 3D OPACC and incubated for 72 hours. Staining was performed using a live and dead cell imaging kit. The cell adherence was observed under a fluorescence microscope. In both experiments, commercial implants under the trade name of Synpor and MedPor were used as comparison. Regardless of the concentration, the viability of the cells treated with 3D OPACC were more than 80%. Besides exhibiting higher cell viability as compared to the commercial implant, the cells also adhered well to the 3D OPACC implant, which indicates that 3D OPACC could be a suitable host for osteoblast attachment and proliferation.