Effect of Double Antibiotic Paste and Calcium Hydroxide on the Viability of the Stem Cells and Their Attachment to the Irrigated Dentin; An In-vitro Study
Samiya Riaz, Aung Thu Htun, Ahmad Azlina
Successful regenerative endodontics in immature permanent teeth is highly dependent on proper disinfection. Intra-canal medicaments used for these procedures should be bactericidal and also provide a biological environment for the viability of stem cells and root maturation. This study was conducted to compare the effects of calcium hydroxide (Ca(OH)2) and double antibiotic paste (DAP) on dental pulp stem cells (DPSCs) viability when seeded on irrigated radicular dentin. Thirty dentin samples were irrigated with 2.5% sodium hypochlorite followed by normal saline (NaCl) rinse. Group-1 samples (n=10) were treated with DAP (1mg/ml) while, group-2 (n=10) with conventional Ca(OH)2 (500mg/ml) and group-3 (n=10) were controls treated with sterile water irrigation only. After 4-weeks of treatment, the samples were rinsed with NaCl. DPSCs from passage 7-9 were seeded onto the samples. Presto blue viability assay was performed on day 1, 3 and 7. Data was analysed using SPSS version 22 (IBM, USA) through one-way ANOVA test. Scanning electron microscope (SEM) imaging was performed on day 7 of cell seeding. Ca(OH)2 group significantly increased DPSCs viability as compared to the DAP group (p<0.05). De-mineralisation of dentin and release of growth factors by Ca(OH)2 promoted the viability of the DPSCs. Therefore, long-term treatment with Ca(OH)2 promotes DPSCs survival and attachment after seeding on irrigated radicular dentin.