Microscopic characterization of two black Aspergillus, A. niger and A. aculeatus from different substrates and indoor environment

Wardah Abdul Rahman and Latiffah Zakaria

  • Latiffah Zakaria


Black Aspergillus occurs in various types of substrates including indoor environment. In the present study, two species of black Aspergillus, A. niger and A. aculeatus were recovered from rice, groundnuts, spices, corn grains, agricultural soil and indoor environment. Both species were identified based on dark or black colony appearance and microscopic characteristics. Microscopic characteristics were observed using Scanning Electron Microscope, as well as light microscope. The characters to differentiate between A. niger and A. aculeatus were the formations of phialides and the shape and size of conidia. Aspergillus niger produced biseriate phialides and globose to subglobose conidia. As for A. aculeatus, the phialides were uniseriate, and the conidia were slightly brown, frequently ellipsoidal with echinulate and evenly spaced spines. Globose to subglobose conidia were also observed. Microscopic characterization is still important, not only to sort the isolates into their respective groups or sections, but it is relatively rapid identification to obtain faster results on the species identity

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