EFFECT OF DEHYDROTHERMAL (DHT) TREATMENT ON THE PHYSICOCHEMICAL PROPERTIES OF 3D POROUS CHITOSAN-COLLAGEN-GLYCERINE SCAFFOLD FOR POTENTIAL SKIN REGENERATING TEMPLATE APPLICATIONS
The aims of this study are to develop 3D porous scaffolds from the blending of chitosan, fish scales collagen and glycerine using freeze dry technique and to investigate the effects of dehydrothermal (DHT) treatment on the physicochemical of the scaffolds. The tensile properties of the scaffold were determined using universal testing machine (UTM) while the porosity and degradation rate of the scaffolds were investigated by using common procedures. The crosslinking density was calculated using 2,4,6-trinitrobenzenesulfonic acid (TNBS) assay while the crosslinking reactions were monitored by Fourier transform infrared (FTIR) technique. Scanning electron microscope (SEM) was used to observe the morphology of the 3D porous structure. The results showed that the 3D scaffolds were porous with interconnected pores having pore size between 100 to 200 microns. The ester and amine absorption band were observed in the FTIR spectrum due to crosslink reaction between functional groups of collagen, glycerine and chitosan. The tensile strength of scaffolds increased with increasing DHT temperature for the exposure time of 24 to 48 hours. The highest strength was achieved on DHT treatment at 120oC for 48 hours i.e. 0.56 ± 0.04 MPa. The degradation rate of scaffold decreased after DHT treatment for all temperatures and times. In conclusion, the 3D scaffolds treated with DHT exhibited excellent stability with sufficient mechanical strength and pore size making them suitable for skin regenerating template applications. The roles of glycerine to facilitate the crosslinking between chitosan and collagen were successfully investigated.