HISTOLOGICAL EFFECT OF HIGH DOSE Aquilaria subintegra LEAVES AQUEOUS EXTRACT ON SEVERAL ORGANS IN ICR MICE
The study was conducted to investigate the histological effects of agarwood, or specifically Aquilaria subintegra aqueous extract (ASAE) leaves on several organs in ICR mice. An acute toxicity study of ASAE was studied. 39 male and 39 female mice aged 12 weeks old were used. The mice were divided into seven groups, and each group contained only twelve mice (six males and six females). All mice orally received the ASAE at a specific concentration of 4000, 5000, 6000, 7000, 8000, 9000 mg/kg body weight, and only one group was used as control which was treated with normal saline. The extract was administered only once on the first day. Male groups 4000, 5000, 7000, 8000 and 9000 mg/kg showed a significant difference in their body weight. There were also some signs of abnormality in mice motility and fur growth in all treated mice. Female face fur group 4000, 5000, 7000, and 9000 mg/kg appeared to be balding, and drowsiness was detected in all treated mice. Liver, kidney and stomach tissues were also affected. Liver’s portal vein in male group 6000 mg/kg and female group 4000 mg/kg were seen filled with blood. All treated mice glomerulus located in kidney expanded and stomach mucosa length also reduced. Based on the data obtained, Aquilaria subintegra, if taken at a dosage of 4000 mg/kg, is toxic to the ICR mice in this study due to the harmful effect it caused on mice appearance, behaviour and organ tissues.