ULTRASTRUCTURAL STUDIES OF SKIN GRAFTS CRYOPRESERVED IN α HELIX ANTARCTIC YEAST ORIENTED ANTI-FREEZE PEPTIDE (Afp1m)

M. S. Khan, M. B. A. Rahman, A.B. MohdZuki , M. M. Noordin and M. Y. Loqman*

  • Loqman Mohamad Yusof

Abstract

ULTRASTRUCTURAL STUDIES OF SKIN GRAFTS CRYOPRESERVED IN α HELIX ANTARCTIC YEAST ORIENTED ANTI-FREEZE PEPTIDE (Afp1m)

S. Khan a,e, M. B. A. Rahman d, A. B. MohdZuki c , M. M. Noordin b and M. Y. Loqman a,*

aDepartment of Companion Animal Medicine and Surgery, Faculty of Veterinary Medicine, Universiti Putra Malaysia

bDepartment of Veterinary Pathology and Microbiology, Faculty of Veterinary Medicine, Universiti Putra Malaysia,

cDepartment of Pre-Clinical Veterinary Studies, Faculty of Veterinary Medicine, Universiti Putra Malaysia,

dDepartment of Chemistry, Faculty of Science, Universiti Putra Malaysia.

eDepartment of Basic Veterinary Sciences, Faculty of Veterinary and animal  Sciences, Gomal University D.I.Khan, Pakistan.

 

Abstract

Livings being of the Antarctic region develop an adaptation to the sub-zero weather conditions by means of anti-freeze proteins (AFP). Anti-freeze peptide (Afp1m) has been synthesized in the sequence structure to similar to the inherent AFP. The objectives of this study were to assess the ultra-structural changes in the Afp1m cryopreserved skin graft using transmission electron microscope (TEM). In the present study, cryopreserve Sprague-Dawley (SD) rat skin grafts with Afp1m, a-helix peptide fragment derived from Glaciozyma antractica yeast were evaluated by its post cryopreservation ultrastructure using TEM to assess its viability. This study also described the damages caused by sub-zero temperatures of -10 and -20°C in cryopreserved tissue using different Afp1m concentrations i.e. 2, 5 and 10 mg/mL for 72 h. TEM examination on tissues cryopreserved in different concentrations of Afp1m showed significant difference in the cryo-damages in the tissues showing less ultra-structural changes at -10 °C as compared to -20°C. In conclusion, various ultra-structural changes in the cryopreserved skin graft were confirmed by TEM in epidermis and underlying connective tissue contents of the dermis in Afp1m cryopreserved skin graft at -20°C.

Keywords: Ultrastructural, Skin Grafts, Yeast, Anti-Freeze Peptide (Afp1m).

ARTICLE INFO

Received 5th November 2017

Received in revised form 13th December 2017

Accepted 18th December 2017

*Corresponding author: Loqman Mohamad Yusof

Tel: +603-79676678 e-mail: loqman@upm.edu.my

Malaysian Journal of Microscopy (2017) ISSN: 1823-7010 eISSN 2600-7444

Published
2017-12-31
Section
Original Research Articles