NEURONAL CELL COUNT AND MORPHOMETRIC STUDY OF HIPPOCAMPAL CA1 PYRAMIDAL NEURONS AFTER CHRONIC NIGELLA SATIVA ADMINISTRATION

N. L. Mohd Yusoff, M. K. A. Sahak, S. Y. X Tiong, and D. S. Hasan Adli*

  • Durriyyah Sharifah Hasan Adli

Abstract

NEURONAL CELL COUNT AND MORPHOMETRIC STUDY OF HIPPOCAMPAL CA1 PYRAMIDAL NEURONS AFTER CHRONIC NIGELLA SATIVA ADMINISTRATION

L. Mohd Yusoff, M. K. A. Sahak, S. Y. X Tiong, and D. S. Hasan Adli*

Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur 50603, Malaysia.

 

Abstract

 

Nigella sativa, a commonly consumed natural supplement, is known for its antioxidant and neuroprotective effects. Following the previous studies which showed its positive effects on spatial memory performance, the current study focused on the neurohistological effect of Nigella sativa on hippocampal cornu ammonis 1 (CA1) pyramidal neurons. The hippocampus is important for memory formation; thus, it is essential to elucidate the characteristics of its constituent neurons. Male Sprague Dawley rats were chronically force-fed for five days a week for 20 consecutive weeks (100 days) with Nigella sativa oil (NSO) (6.0 l/100 g body weight) and corn oil (CO) (0.1 ml/100 g body weight) as control. Dorsal hippocampal transverse sections (10 m thick) from both groups were visualized under the light microscope and morphometric analysis was conducted using the Image-Pro Premier 9.1 64-bit software. Only cresyl-violet stained neuronal somas with each having whole visible nucleus and nucleolus were included in the morphometric analysis. Mean number of neuronal cell count in NSO group was found to be higher than the controls. Significant differences in somatic area/SA, somatic perimeter/SP, somatic aspect ratio/SAR, somatic circularity index/SCI, and somatic roundness/SRo were observed between the groups. Values of two parameters (SA and SP) of NSO group indicated significantly larger sized CA1 neurons, whereas values of the other three parameters (SAR, SCI and SRo) significantly indicated less round in shape CA1 neurons. These distinct effects on the morphological characteristics could be due to NSO various biochemical constituents.

Keywords: Morphometric, hippocampal neurons, Nigella sativa.

ARTICLE INFO

Received 13th November 2017

Received in revised form 8th December 2017

Accepted 9th December 2017

*Corresponding author: Durriyyah Sharifah Hasan Adli

Tel: +6(03)79674213 e-mail: durriyyah@um.edu.my

Malaysian Journal of Microscopy (2017) ISSN: 1823-7010 eISSN 2600-7444

Published
2017-12-31
Section
Original Research Articles