MORPHOLOGICAL CHANGE OF HUMAN EXFOLIATED DECIDUOUS TEETH AND THE EFFECT OF VASCULAR ENDOTHELIAL GROWTH FACTOR ON HUMAN AMNIOTIC MEMBRANE SCAFFOLD

Md Hashim SN, Yusof MFH, Alshehadat SA, Kannan TP, Suzina SAH, Mokhtar KI and Azlina A*

  • Azlina A

Abstract

MORPHOLOGICAL CHANGE OF HUMAN EXFOLIATED DECIDUOUS TEETH AND THE EFFECT OF VASCULAR ENDOTHELIAL GROWTH FACTOR ON HUMAN AMNIOTIC MEMBRANE SCAFFOLD

 

Md Hashim SN1, Yusof MFH1, Alshehadat SA1, Kannan TP1,2, Suzina SAH3, Mokhtar KI4 and Azlina A1[1]

1School of Dental Sciences, 2Human Genome Centre, School of Medical Sciences, Universiti Sains Malaysia,

16150 Kubang Kerian, Kelantan, Malaysia.

3Tissue Bank, School of Medical Sciences, Universiti Sains Malaysia,

16150 Kubang Kerian, Kelantan, Malaysia.

4Kulliyyah of Dentistry, International Islamic University Malaysia,

25200 Kuantan, Pahang, Malaysia.

 

Angiogenicity is one of the essential components to enable tissue function. It is important to develop a construct that would help in catering oxygen and nutrient to the engineered tissue area. Thus, this study aims to investigate the attachment, spreading and growth of stem cells from human exfoliated deciduous teeth (SHED) on human AM (HAM) with or without vascular endothelial growth factor (VEGF) using scanning electron microscope (SEM), and indirectly see the potential of the HAM as a scaffold to promote angiogenic micro-environment. Since day 1, there were continuous changes of the cell morphology until day 28, SHED treated with VEGF seemed to change its shape from fibroblast-like into a round-shape cell, similar structure as an endothelial-like cell. The structures of filopodia-like were also observed on the treated SHED. SHED without VEGF treatment showed only normal morphological growth on HAM. VEGF is a protein produced to stimulate angiogenesis, and is believed to contribute to the morphological changes of SHED seeded on HAM. This indicates that HAM could be used as a scaffold to allow SHED differentiation into endothelial-like cells with the induction of VEGF.

 

Keywords: amniotic membrane, vascular endothelial growth factor, stem cells from human exfoliated deciduous teeth

 

[1] Corresponding author: Tel: +609-767 5827

 E-mail: azlinakb@usm.my

Published
2015-12-31
Section
Original Research Articles