PHYSICAL CHARACTERIZATION OF GREEN SYNTHESIZED SILVER NANOPARTICLES USING BROWN SEAWEED SARGASSUM BINDERI

Yu  -Ke Xin; Divashini  Navaneetharajah; Nurshahida Amat; Norliza Zulkifli; Ng  Chean Hui; Shahida  Mohd Said; Shirley Gee Hoon Tang

Yu -Ke Xin Management and Science University https://orcid.org/0000-0003-4923-1929
Divashini Navaneetharajah Management and Science University
Nurshahida Amat Management and Science University
Norliza Zulkifli Management and Science University
Ng Chean Hui Management and Science University https://orcid.org/0000-0002-1474-6976
Shahida Mohd Said Universiti Kebangsaan Malaysia https://orcid.org/0000-0003-4387-1087
Shirley Gee Hoon Tang Universiti Kebangsaan Malaysia https://orcid.org/0000-0002-8101-7204

Keywords: Sargassum binderi, silver nanoparticles, green synthesis, physical characterization

Abstract

Green synthesized silver nanoparticles are new in nanotechnology, proven to be eco-friendly and cost effective. Nanoparticles derived from seaweeds have high value in biomedical field. This study was aimed to characterize the green-synthesized silver nanoparticles (AgNPs) using brown seaweed Sargassum binderi. A total of 10 g dried fine cut seaweed S. binderi was boiled in 100 mL distilled water to light yellow colour. The extract was filtered and centrifuged. The pellet of extract was freeze-dried. Synthesis of AgNPs was done by mixing seaweed extract (0.08 mg/ml) with silver nitrate (2.0 mM) at pH 9, room temperature, in 20 minutes. The solution was centrifuged to obtain the pellet, and it was oven-dried for 24 hours to yield dry form of AgNPs. Total of 4.4 mg dark-coloured AgNPs using seaweed S. binderi was synthesized. UV-vis analysis (ranged 200-600 nm) of S. binderi extract showed peak at 305 nm while AgNPs formation at peak 425 nm. FTIR analysis (ranged 4000–400 cm−1) of S. binderi extract showed peaks at 3347, 1642, 672 and 600 cm−1, while AgNPs showed peaks at 3350, 1650, 680 and 600 cm−1. SEM revealed spherical shape of AgNPs with the size of 39.63 ± 9.72 nm in diameter. EDX spectrum illustrated elemental constituents of silver (82.3%), carbon (8.5%), oxygen (7.9%) and nitrogen (0.3%). Quantitative measurements have shown the parameter setting of AgNPs synthesis is valid. Results indicate the potential use of brown seaweed S. binderi in the synthesis of AgNPs which has enormous possibilites in biomedical applications.